use only. Fibrinogen Is Not Synthesized by Human Megakaryocytes
نویسندگان
چکیده
http://bloodjournal.hematologylibrary.org/site/misc/rights.xhtml#repub_requests Information about reproducing this article in parts or in its entirety may be found online at: http://bloodjournal.hematologylibrary.org/site/misc/rights.xhtml#reprints Information about ordering reprints may be found online at: http://bloodjournal.hematologylibrary.org/site/subscriptions/index.xhtml Information about subscriptions and ASH membership may be found online at:
منابع مشابه
RAPID COMMUNICATION Fibrinogen y-Chain mRNA Is Not Detected In Human Megakaryocytes
Human megakaryocytes and platelets contain counterparts of several plasma proteins. The origin of most of these a-granule proteins is unclear. Fibrinogen represents one of those molecules, being essential in hemostasis, thrombosis, and platelet aggregation. To study whether fibrinogen is endocytosed by megakaryocytes and packaged into a-granules or newly synthesized by these cells, we establish...
متن کاملFibrinogen is not synthesized by human megakaryocytes.
The origin of platelet fibrinogen is controversial. It may arise from two sources: (a) exogenously by endocytosis of plasma fibrinogen, or (b) endogenously by synthesis. We explored the second possibility because we previously demonstrated that the first mechanism does occur. Fibrinogen synthesis by human megakaryocytes (MK) was investigated by in situ hybridization and the polymerase chain rea...
متن کاملFibrinogen biosynthesis in isolated guinea pig megakaryocytes.
Fibrinogen synthesis was investigated in guinea pig megakaryocytes. Purified megakaryocytes were incubated with 35S-methionine in methionine-free incubation medium for 18 hours. Newly synthesized fibrinogen in megakaryocyte lysates enriched with purified carrier guinea pig fibrinogen was immunoprecipitated with a specific anti-guinea pig fibrinogen antiserum produced in rabbits. Proteins in the...
متن کاملEndocytosis of Fibrinogen Into Hamster Megakaryocyte
Two species of fibrinogen that differ only in the structure of their y chains, yA and y', are present in normal plasma. Fibrinogen stored in platelet a granules does not contain y' chains. Because platelet fibrinogen was recently shown to be derived exclusively by receptor-mediated endocytosis from plasma and not by endogenous megakaryocyte synthesis, we postulated that the y' fibrinogen presen...
متن کاملPlatelet a - Granule Fibrinogen , Albumin , and Immunoglobulin G Are Not Synthesized by Rat and Mouse Megakaryocytes
It has been assumed that endogenous synthesis by the platelet precursor cell, the bone marrow megakaryocyte, is the major source of platelet a-granule protein. To test this hypothesis, we used mRNA phenotyping to detect in megakaryocytes the presence of mRNA transcripts specific for various proteins. Our results indicate that megakaryocytes synthesize platelet factor 4, a protein relatively spe...
متن کامل